The effect of transforming growth factor-beta1 on EDA region of fibronectin in oral squamous cell carcinoma and adenoid cystic carcinoma cells / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of transforming growth factor-beta1 (TGF-beta1) on EDA region of fibronectin in oral squamous cell carcinoma and adenoid cystic carcinoma cells.</p><p><b>METHODS</b>Immunohistochemistry and reverse transcription polymerase chain reaction technique were used to detect the changes of EDA proteins and mRNAs expression.</p><p><b>RESULTS</b>The immunostaining ratio in Tca83 cells with TGF-beta1 was greatly higher than that in Tca83 cells without TGF-beta1 (P < 0.01), the immunostaining ratio in SACC-83 cells with TGF-beta1 was higher than that in SACC-83 cells without TGF-beta1 (P < 0.05), but there was no significant difference between SACC-LM cells with TGF-beta1 and SACC-LM cells without TGF-beta1 (P > 0.05). In the three oral carcinoma cells, the expression of EDA(+) mRNAs in the group with TGF-beta1 was higher than that in the group without TGF-beta1 (P < 0.05). The expression of EDA(-) mRNAs in the group with TGF-beta1 was lower than that in the group without TGF-beta1 (P < 0.05).</p><p><b>CONCLUSIONS</b>TGF-beta1 could influence EDA region splicings and upregulate the expression of EDA region in Tca83, SACC-83 and SACC-LM cells, and might play an important role in accelerating oral carcinoma cells adhesion and tumor invasion and metastasis.</p>