Effect of verapamil on the proliferation of normal gingival fibroblast / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the effect of verapamil on the proliferation of normal gingival fibroblast (NGF) in vitro.</p><p><b>METHODS</b>NGF was isolated and cultured. The 5th passage of NGF was incubated with 0, 0.1, 1, 10, 100 micromol/L verapamil respectively. Methyl thiazolyl tetrazolium (MTT) assay and flow cytometry were used to detect cell proliferation and cell cycles.</p><p><b>RESULTS</b>Incubated with 100 micromol/L verapamil for 66 h, the A value of normal gingival fibroblast was significantly lower than those without verapamil groups (P < 0.01). Incubated with 100 micromol/L verapamil for 18 h, 69% of cells were at the G(0) - G(1) phase, 27% were at the S phase. For control group (without verapamil) 41% of cells were at G(0) - G(1) phase and 49% cells were at S phase. There was significant difference between the two groups (P < 0.001).</p><p><b>CONCLUSIONS</b>100 micromol/L verapamil inhibited proliferation of normal gingival fibroblast by a cell-cycle arrest.</p>