MT1-MMP up-regulates VEGF expression in human breast carcinoma MCF-7 cells and induces tumor angiogenesis / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 727-731, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-293065
ABSTRACT
<p><b>OBJECTIVE</b>To study the function of MT1-MMP in tumor angiogenesis and elucidate the possible way of action that MT1-MMP contributes to angiogenesis.</p><p><b>METHODS</b>MT1-MMP was transfected into human breast carcinoma cell line MCF-7 cells. Semi-quantitative RT-PCR and immunofluorescence staining were employed to detect the expression of VEGF in the transfected and non-transfected MCF-7 cells. Tumor growth, microvessel density and expression of VEGF in nude mice were detected through in vivo tumorigenicity assay.</p><p><b>RESULTS</b>In MT1-MMP stable transfected MCF-7 cells, mRNA expression of VEGF(189), VEGF(165), and VEGF(121) and immunofluorescence intensity were significantly elevated (P < 0.001). In vivo tumorigenicity assay in the nude mice showed that MT1-MMP promoted tumor growth. The MVD in the MT1-MMP-transfected cells-transplanted tumor tissue was significantly elevated (P < 0.05). Immunohistochemical assay showed that there was a strong immunostaining of VEGF in those tumor tissues.</p><p><b>CONCLUSION</b>MT1-MMP can induce tumor angiogenesis through up-regulation of VEGF expression. This function of MT1-MMP may open a new approach for clinical anti-tumor research and anti-tumor drug development.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Breast Neoplasms
/
RNA, Messenger
/
Gene Expression Regulation, Neoplastic
/
Up-Regulation
/
Cell Line, Tumor
/
Vascular Endothelial Growth Factor A
/
Tumor Burden
/
Matrix Metalloproteinase 14
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2009
Type:
Article
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