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MT1-MMP up-regulates VEGF expression in human breast carcinoma MCF-7 cells and induces tumor angiogenesis / 中华肿瘤杂志
Chinese Journal of Oncology ; (12): 727-731, 2009.
Article in Chinese | WPRIM | ID: wpr-293065
ABSTRACT
<p><b>OBJECTIVE</b>To study the function of MT1-MMP in tumor angiogenesis and elucidate the possible way of action that MT1-MMP contributes to angiogenesis.</p><p><b>METHODS</b>MT1-MMP was transfected into human breast carcinoma cell line MCF-7 cells. Semi-quantitative RT-PCR and immunofluorescence staining were employed to detect the expression of VEGF in the transfected and non-transfected MCF-7 cells. Tumor growth, microvessel density and expression of VEGF in nude mice were detected through in vivo tumorigenicity assay.</p><p><b>RESULTS</b>In MT1-MMP stable transfected MCF-7 cells, mRNA expression of VEGF(189), VEGF(165), and VEGF(121) and immunofluorescence intensity were significantly elevated (P < 0.001). In vivo tumorigenicity assay in the nude mice showed that MT1-MMP promoted tumor growth. The MVD in the MT1-MMP-transfected cells-transplanted tumor tissue was significantly elevated (P < 0.05). Immunohistochemical assay showed that there was a strong immunostaining of VEGF in those tumor tissues.</p><p><b>CONCLUSION</b>MT1-MMP can induce tumor angiogenesis through up-regulation of VEGF expression. This function of MT1-MMP may open a new approach for clinical anti-tumor research and anti-tumor drug development.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Physiology / Breast Neoplasms / RNA, Messenger / Gene Expression Regulation, Neoplastic / Up-Regulation / Cell Line, Tumor / Vascular Endothelial Growth Factor A / Tumor Burden / Matrix Metalloproteinase 14 Limits: Animals / Female / Humans Language: Chinese Journal: Chinese Journal of Oncology Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Physiology / Breast Neoplasms / RNA, Messenger / Gene Expression Regulation, Neoplastic / Up-Regulation / Cell Line, Tumor / Vascular Endothelial Growth Factor A / Tumor Burden / Matrix Metalloproteinase 14 Limits: Animals / Female / Humans Language: Chinese Journal: Chinese Journal of Oncology Year: 2009 Type: Article