Study on effect of fushenkeli on expression of TAK1 in human renal tubular epithelial cells and its possible mechanism / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 2571-2575, 2011.
Article
in Zh
| WPRIM
| ID: wpr-293200
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of fushenkeli on the expression of TAK1 in the proliferation of the renal tubular epithelial cells induced by TGF-beta1 and its possible mechanism.</p><p><b>METHOD</b>Human renal tubular epithelial (HK-2) cells were divided into five groups:blank control group, TGF-beta1 group (5 microg x L(-1)), intervention group 1 (5 microg x L(-1) of TGF-beta1 + 100 mg x L(-1) of fushenkeli), intervention group 2 (5 microg x L(-1) of TGF-beta1 + 500 mg x L(-1) of fushenkeli) and intervention group 3 (5 microg x L(-1) of TGF-beta1 + 1 g x L(-1) of fushenkeli). HK-2 proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay. Type IV collagen in the supernatants of the cultured HK-2 was detected by ELISA at 12, 24, 48 hours respectively. The protein and mRNA expressions of TAK1 was measured by Western blot and real-time quantitative PCR.</p><p><b>RESULT</b>1) The cell proliferation and the expression of type IV collagen were increased compared with the control group (P<0.05, P<0.01), but they were decreased in intervention group. 2) The expressions of protein and mRNA of TAK1 in TGF-beta1 group were upregulating significantly compared with control group (P<0.01), but they were downregulating in intervention group, especially in intervention group 3.</p><p><b>CONCLUSION</b>Fushenkeli could inhibits TAK1 expression induced by TGF-beta1 in the proliferation of HK-2 cell.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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RNA, Messenger
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Drugs, Chinese Herbal
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Cell Line
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MAP Kinase Kinase Kinases
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Cell Biology
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Collagen Type IV
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Cell Proliferation
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Epithelial Cells
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Transforming Growth Factor beta1
Limits:
Humans
Language:
Zh
Journal:
Zhongguo Zhong Yao Za Zhi
Year:
2011
Type:
Article