Anti-HIV activity and mechanism of Cynanchum otophyllum glucan sulfate in vitro / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study anti-HIV activity and mechanism of Cynanchum otophyllum glucan sulfate in vitro.</p><p><b>METHOD</b>Anti-HIV-1 activity was detected with syncytial formation assay and quantitative P24 enzyme-linked immunosorbent assay (ELISA); cytotoxicity was tested with MTT colorimetric assay. Antiviral mechanism was investigated by fusion inhibition, time of addition and pre-treatment experiments.</p><p><b>RESULT</b>The 50% inhibition concentrations (IC50) of PS20 for HIV-1(IIIB), HIV-1(Ada-M), and HIV-1(Bal), were 0.26, 0.46, 0.90 micromol x L(-1), respectively. Studies on antiviral mechanism of PS20 showed that target molecule may be viral envelope protein.</p><p><b>CONCLUSION</b>The results suggested that PS20 had high anti-HIV activity and was worth to be studied further.</p>