Pollen germination in vitro and pollen tube growth of Scutellaria baicalensis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2636-2640, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-293248
ABSTRACT
<p><b>OBJECTIVE</b>To study the pollen germination in vitro and pollen tube growth of Scutellaria baicalensis.</p><p><b>METHOD</b>Two years-old S. baicalensis that in the experiment field of Shangluo university were used as the materials. The effects of culture medium, sucrose, PEG, pH value, culture time and temperature on the pollen germination and tube growth of S. baicalensis were investigated using the method of liquid culture.</p><p><b>RESULT AND CONCLUSION</b>The results showed that 100 g x L(-1) sucrose was helpful for the germination and growth of pollens. However, higher concentration of sucrose would inhibit strongly pollen germination and the growth of pollen tubes. PEG could accelerate pollen germination and tube growth, and the optimal concentration was 150 g x L(-1). The optimum culture medium was BK and BK + 10% sucrose + 150 g x L(-1) PEG (pH 5.8) , and the latter was more benefit for pollen germination and tube growth. The pollen of germination was better at 25-35 degrees C, and it was the best at 30 degrees C for 2-3 hours, while pollen tube growth was slower at 25 degrees C.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Temperature
/
Cells, Cultured
/
Germination
/
Cell Culture Techniques
/
Culture Media
/
Scutellaria baicalensis
/
Pollen Tube
/
Hydrogen-Ion Concentration
/
Metabolism
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article
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