Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 618-623, 2014.
Article
in English
| WPRIM
| ID: wpr-293262
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the possible mechanism through which Artemisinin induced apoptosis in pancreatic cell line.</p><p><b>METHODS</b>Column chromatography, thin layer chromatography (TLC) and proton NMR spectroscopy were used to purify Artemisinin. The flowcytometry was employed to detect apoptosis and reactive oxygen species (ROS).</p><p><b>RESULTS</b>The results indicated that 50% inhibiting concentration (IC50 value) for pancreatic cell line (RIN) was 45 μmol/L of Artemisinin. Artemisinin had no cytotoxic effect on the growth of peripheral blood lymphocytes. The mechanism of apoptosis was evaluated by measuring intracellular ROS. It was shown that Artemisinin-induced apoptosis occurred independently of the binding of CD95L to CD95 receptor in the RIN cells. Moreover, Artemisinin, in a dose-dependent manner, could significantly increase the level of ROS.</p><p><b>CONCLUSION</b>Artemisinin can induce apoptosis in the RIN cells via the generation of ROS and triggering the intrinsic pathway of cell death.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreatic Neoplasms
/
Pathology
/
Pharmacology
/
Propidium
/
Time Factors
/
Reactive Oxygen Species
/
Apoptosis
/
Annexin A5
/
Colorimetry
/
Fas Receptor
Limits:
Humans
Language:
English
Journal:
Chinese journal of integrative medicine
Year:
2014
Type:
Article
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