Baicalin attenuates focal cerebral ischemic reperfusion injury by inhibition of protease-activated receptor-1 and apoptosis / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 116-122, 2014.
Article
in English
| WPRIM
| ID: wpr-293288
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the neuro-protective effects of baicalin in Wistar rats with focal cerebral ischemic reperfusion injury.</p><p><b>METHODS</b>Ninety adult male Wistar rats weighing 320-350 g were randomly divided into the following groups (n=5) (a) sham control group; (b) vehicle group, subjected to middle cerebral artery occlusion and received vehicle intraperitoneally; (c-e) baicalin groups, which were subjected to the middle cerebral artery occlusion and treated with baicalin 25, 50 and 100 mg/kg, respectively. The neurological scores were determined at postoperative 1, 3 and 7 d after the treatment. The expression of protease-activated receptor-1 (PAR-1), PAR-1 mRNA and Caspase-3 were determined using Western blot, reverse transcription polymerase chain reaction (RTPCR) analysis and immunohistochemistry, respectively.</p><p><b>RESULTS</b>Significant decrease was noted in the neurological score in the baicalin group compared with that of the vehicle group (P<0.01). Additionally, down-regulation of PAR-1 mRNA, PAR-1 and Caspase-3 was observed in the baicalin groups compared with those obtained from the vehicle group (P<0.01). Compared with the low-dose baicalin group (25 mg/kg), remarkable decrease was noted in neurological score, and the expression of PAR-1 mRNA, PAR-1 as well as Caspase-3 in the high-dose group (P<0.05).</p><p><b>CONCLUSION</b>Baicalin showed neuro-protective effects in focal cerebral ischemic reperfusion injury through inhibiting the expression of PAR-1 and apoptosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Flavonoids
/
RNA, Messenger
/
Reperfusion Injury
/
Brain Ischemia
/
Gene Expression Regulation
/
Rats, Wistar
/
Apoptosis
/
Receptor, PAR-1
Limits:
Animals
Language:
English
Journal:
Chinese journal of integrative medicine
Year:
2014
Type:
Article
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