Expression of peroxisome proliferator-activated receptor gamma in glioma / 南方医科大学学报
Journal of Southern Medical University
;
(12): 444-446, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-293358
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and significance of peroxisome proliferators-activated receptor gamma (PPAR gamma) in human glioma.</p><p><b>METHODS</b>Immunohistochemical staining for PPAR gamma was performed using biopsy specimens of human glioma of various histological types. Expression of PPAR gamma and GFAP in glioma cell lines SWO-38, U251 and SHG-44 were analyzed using Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Immunohistochemical study showed that PPAR gamma was expressed in glioma tissues with positive rate of 37.5%. Western blotting and RT-PCR showed that PPAR gamma was expressed in both glioma cell lines SWO-38 and U251, but not in SHG-44 cells. However, high expression of GFAP was detected in SHG-44 cells.</p><p><b>CONCLUSION</b>PPAR gamma is associated with carcinogens of glioma. Actived PPAR gamma by agonist may be a novel approach to the treatment of glioma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Brain Neoplasms
/
RNA, Messenger
/
Immunohistochemistry
/
Blotting, Western
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Line, Tumor
/
PPAR gamma
/
Genetics
/
Glial Fibrillary Acidic Protein
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article
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