Study on ligustrazine in reversing multidrug resistance of HepG2/ADM cell in vitro / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 970-973, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-293659
ABSTRACT
<p><b>OBJECTIVE</b>To study the reverse effect of ligustrazine (TMP) on HepG2/ADM, a herd of hepatocellular carcinoma cell, multidrug resistance (MDR) and the influence of P-gp170 expression.</p><p><b>METHOD</b>The reverse effect of ligustrazine on HepG2/ADM cell was observed, with the methods of cell culture, MTT's analyze, RT-PCR and Flow cytometric, etc.</p><p><b>RESULT</b>Ligustrazine could make MDR of cell line of HepG2/ADM reduce the expression of P-gp170, enhance the density of adriamycin in cell and increase the adriamycin's cytotoxicity. With the Flow cytometric, the results of RT-PCR showed the transcriptional activity of the MDR1 decreased.</p><p><b>CONCLUSION</b>Ligustrazine can reverse MDR of HCC cell line of HepG2/ADM and has prospect in clinical use.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Pyrazines
/
Calcium Channel Blockers
/
Glycoproteins
/
Doxorubicin
/
Carcinoma, Hepatocellular
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
Genes, MDR
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2004
Type:
Article
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