Study of the methods of determining the isoflavones in herbs of Trifolium pratense extractive / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the methods of determining the amount of Isoflavones in red clover extractive.</p><p><b>METHOD</b>RP-HPLC is employed to determine the Isoflavones, with a C18 RP Column, a moble phase of MeOH-CH3CN-0.1%H3PO4 and a detection wavelength of 260 nm.</p><p><b>RESULT</b>After being water-solved, the four groups of flavoes elements satisfactorily separated, the amount of feeding in a range of 0.024-0.336 microg which has a good linear relationship with the peaks, and the total isoflavones determining results repetition, RSD=10.1%.</p><p><b>CONCLUSION</b>A simple, reliable and effective quality-control method is given.</p>