Induction of cell damage and change of miR-133b expression by paraquat in PC12 cells / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 2-6, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-293770
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of paraquat on induction of cell damage and miR-133b expression in PC12 cells.</p><p><b>METHODS</b>Cytotoxicity of PC12 cells was measured by MTT assay, following the PC12 cells treatment with 50, 100, or 300 µmol/L paraquat. Cell apoptosis was examined by the method of Annexin V-FITC/PI in flow cytometry (FCM) and the relative level of miR-133b expression was measured by real time RT-PCR, following the PC12 cells treatment with 100 or 300 µmol/L paraquat.</p><p><b>RESULTS</b>Survival rate of PC12 cells treated with 100 or 300 µmol/L paraquat was lower than that of the vehicle control group (P < 0.01, P < 0.05), in the dose dependent pattern. Apoptotic rate of PC12 cells treated with 100, 300 µmol/L paraquat was higher than that of the vehicle control group (P < 0.05). The relative level of miR-133b expression of PC12 cells treated with 300 µmol/L paraquat was higher than that of the vehicle control group (P < 0.05).</p><p><b>CONCLUSIONS</b>Paraquat may cause cell damage and induce apoptosis in PC12 cells, and induce miR-133b expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Paraquat
/
PC12 Cells
/
Apoptosis
/
MicroRNAs
/
Toxicity
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2011
Type:
Article
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