Development and identification of monoclonal antibodies of cape jasmine proteins in Reduning injection / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1606-1609, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-294059
ABSTRACT
Liposoluble cape jasmine proteins were used to immunize BALB/C mice. Indirect ELISA was utilized to develop one monoclonal antibody by integrating SP2/0 cells and spleen cells from immunized BALB/C mice. The subclass of the monoclonal antibody was identified as IgG2b, with Kappa chain as its light chain. The ascite titer of 2H8 monoclonal antibody was 1204 080. Western-blot analysis proved that 2H8 reacted with cape jasmine proteins to identify specific liposoluble protein with molecuar weight of around 58.5 kDa. Dot-ELISA was established with 2H8 ascites as the primary antibody, showing the minimum detectable amount of 19.5 ng. This study lays a foundation for the development of protein kits of Reduning injection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plant Proteins
/
Reagent Kits, Diagnostic
/
Enzyme-Linked Immunosorbent Assay
/
Drugs, Chinese Herbal
/
Drug Hypersensitivity
/
Allergy and Immunology
/
Methods
/
Mice, Inbred BALB C
/
Antibodies, Monoclonal
/
Antibody Specificity
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article
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