Phosphatidylinositol 3-kinase inhibitor, LY294002, induced senescence-like changes in human diploid fibroblasts / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 901-905, 2003.
Article
in English
| WPRIM
| ID: wpr-294206
ABSTRACT
<p><b>OBJECTIVE</b>To reveal the role of Phosphatidylinositol 3-kinases (PI3Ks) in regulating human diploid fibroblast (2BS cell) senescence as well as the possible mechanisms involved.</p><p><b>METHODS</b>Using a PI3Ks specific inhibitor, LY294002, cell cycle, apoptosis, proliferation, senescence association beta-galactosidase staining as well as senescence association CKIs, p16(INK4) and p21(Cip1) protein expressions were all measured in the low passages of 2BS cells.</p><p><b>RESULTS</b>Both 25 micro mol/L and 50 micro mol/L concentrations of LY294002 could cause a significant decrease in cells entering into S phase, and this cell cycle of G(1) phase arrest was dose-dependent. Meanwhile, LY294002 contributed to apoptosis, caused 2BS cell growth arrest, and activated senescence association beta-galactosidase (P < 0.05). In addition, LY294002 could induce time-course expressions of p16(INK4) and p21(Cip1) in 2BS cell lines.</p><p><b>CONCLUSIONS</b>PI3Ks inhibitor LY294002 could induce senescence-like changes in 2BS cell lines. Two senescence associated CKIs, p16(INK4) and p21(Cip1), might be involved in this senescence phenotype proceeding in 2BS cell lines.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Cells, Cultured
/
Morpholines
/
G1 Phase
/
Chromones
/
Cellular Senescence
/
Cyclins
/
Phosphatidylinositol 3-Kinases
/
Cyclin-Dependent Kinase Inhibitor p16
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2003
Type:
Article
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