Tumor necrosis factor-alpha -308G/A polymorphism and risk of hepatocellular carcinoma in hepatitis C virus-infected patients / 癌症
Chinese Journal of Cancer
; (12): 29-35, 2012.
Article
in En
| WPRIM
| ID: wpr-294440
Responsible library:
WPRO
ABSTRACT
Tumor necrosis factor-alpha (TNF-α) is an important cytokine in generating an immune response against infection with hepatitis C virus (HCV). The functions of TNF-α may be altered by single-nucleotide polymorphisms (SNPs) in its gene structure. We hypothesized that SNPs in TNF-α may be important in determining the outcome of an HCV infection. To test this hypothesis, we investigated the role of the polymorphism -308G/A, which is located in the promoter region of the TNF-α gene, in the progression of HCV infection in Egyptian patients using a quantitative real-time polymerase chain reaction (qRT-PCR). The distribution of this polymorphism and its impact on the serum level of TNF-α was compared between 90 HCV-infected patients [45 with HCV-induced cirrhosis and 45 with HCV-related hepatocellular carcinoma (HCC)] and 45 healthy Egyptian volunteers without any history of liver disease. Our results showed that at the TNF-α -308 position, the G/G allele was most common (78.5%) in the study population, with the G/A and A/A alleles occurring less frequently (13.3% and 8.1%, respectively). Frequencies of G/G, G/A, and A/A genotypes were 87%, 7%, and 6% in patients with liver cirrhosis and were 94%, 4%, and 2% in patients with HCC, respectively. Serum levels of TNF-α were significantly higher in HCV-infected patients than in healthy controls, indicating that the TNF-α -308 polymorphism does not influence the production of TNF-α. The serum level of TNF-α was positively correlated with HCV infection. Taken together, these findings suggest that the TNF-α -308 polymorphism may not be a host genetic factor associated with the severity of HCV infection, but may be an independent risk factor for HCC.
Full text:
1
Index:
WPRIM
Main subject:
Virology
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Blood
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Risk Factors
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Tumor Necrosis Factor-alpha
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Carcinoma, Hepatocellular
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Hepatitis C, Chronic
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Genetic Predisposition to Disease
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Polymorphism, Single Nucleotide
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Alleles
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Real-Time Polymerase Chain Reaction
Type of study:
Etiology_studies
/
Risk_factors_studies
Limits:
Adult
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Aged
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Female
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Humans
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Male
Language:
En
Journal:
Chinese Journal of Cancer
Year:
2012
Type:
Article