Overexpression of centromere protein H is significantly associated with breast cancer progression and overall patient survival / 癌症
Chinese Journal of Cancer
;
(12): 627-637, 2011.
Article
in English
| WPRIM
| ID: wpr-294481
ABSTRACT
Breast cancer is one of the leading causes of cancer death worldwide. This study aimed to analyze the expression of centromere protein H (CENP-H) in breast cancer and to correlate it with clinicopathologic data, including patient survival. Using reverse transcription-polymerase chain reaction and Western blotting to detect the expression of CENP-H in normal mammary epithelial cells, immortalized mammary epithelial cell lines, and breast cancer cell lines, we observed that the mRNA and protein levels of CENP-H were higher in breast cancer cell lines and in immortalized mammary epithelial cells than in normal mammary epithelial cells. We next examined CENP-H expression in 307 paraffin-embedded archived samples of clinicopathologically characterized breast cancer using immunohistochemistry, and detected high CENP-H expression in 134 (43.6%) samples. Statistical analysis showed that CENP-H expression was related with clinical stage (P = 0.001), T classification (P = 0.032), N classification (P = 0.018), and Ki-67 (P < 0.001). Patients with high CENP-H expression had short overall survival. Multivariate analysis showed that CENP-H expression was an independent prognostic indicator for patient survival. Our results suggest that CENP-H protein is a valuable marker of breast cancer progression and prognosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Breast
/
Breast Neoplasms
/
RNA, Messenger
/
Chromosomal Proteins, Non-Histone
/
Up-Regulation
/
Cell Line
/
Blotting, Western
/
Survival Rate
/
Follow-Up Studies
Type of study:
Observational study
/
Prognostic study
/
Risk factors
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
Language:
English
Journal:
Chinese Journal of Cancer
Year:
2011
Type:
Article
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