Overexpression of centromere protein H is significantly associated with breast cancer progression and overall patient survival / 癌症
Chinese Journal of Cancer
; (12): 627-637, 2011.
Article
in En
| WPRIM
| ID: wpr-294481
Responsible library:
WPRO
ABSTRACT
Breast cancer is one of the leading causes of cancer death worldwide. This study aimed to analyze the expression of centromere protein H (CENP-H) in breast cancer and to correlate it with clinicopathologic data, including patient survival. Using reverse transcription-polymerase chain reaction and Western blotting to detect the expression of CENP-H in normal mammary epithelial cells, immortalized mammary epithelial cell lines, and breast cancer cell lines, we observed that the mRNA and protein levels of CENP-H were higher in breast cancer cell lines and in immortalized mammary epithelial cells than in normal mammary epithelial cells. We next examined CENP-H expression in 307 paraffin-embedded archived samples of clinicopathologically characterized breast cancer using immunohistochemistry, and detected high CENP-H expression in 134 (43.6%) samples. Statistical analysis showed that CENP-H expression was related with clinical stage (P = 0.001), T classification (P = 0.032), N classification (P = 0.018), and Ki-67 (P < 0.001). Patients with high CENP-H expression had short overall survival. Multivariate analysis showed that CENP-H expression was an independent prognostic indicator for patient survival. Our results suggest that CENP-H protein is a valuable marker of breast cancer progression and prognosis.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Breast
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Breast Neoplasms
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RNA, Messenger
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Chromosomal Proteins, Non-Histone
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Up-Regulation
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Cell Line
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Blotting, Western
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Survival Rate
/
Follow-Up Studies
Type of study:
Observational_studies
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Prognostic_studies
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Risk_factors_studies
Limits:
Adult
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Aged
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Aged80
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Female
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Humans
Language:
En
Journal:
Chinese Journal of Cancer
Year:
2011
Type:
Article