The effect of co-immobilized TNF-alpha/IFN-gamma on mitochondrial membrane potential of HeLa cells / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 972-977, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-294529
ABSTRACT
This study inquired into the mechanisms of co-immobilized cytokines and free cytokines-induced apoptosis on HeLa cells. With the use of photochemical fixed method, TNF-alpha/IFN-gamma were co-immobilized on a 24-well polystyrene culture plate. HeLa cells were stained with fluorescent probe JC-1 to detect the changes of mitochondrial membrane potential (deltapsim), and then were examined by flow cytometry. The results showed that co-immobilized cytokines could induce the apoptosis of HeLa cells in a dose-independent manner. When treated with low-dose of co-immobilized cytokines (20ng/ml), the mitochondrial membrane potential (deltapsim) of HeLa cells continually decreased in 6 days. These indicate that low dose co-immobilized cytokines have a long-term of apoptosis-inducing effect on HeLa cells. We assume that there is close relationship between the mitochondrial membrane potential decrease and the apoptosis of HeLa cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
HeLa Cells
/
Interferon-gamma
/
Tumor Necrosis Factor-alpha
/
Apoptosis
/
Dose-Response Relationship, Drug
/
Mitochondrial Membranes
/
Membrane Potential, Mitochondrial
/
Immobilized Proteins
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2009
Type:
Article
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