Cloning and expression of extracellular region gene located in N-terminus of Leishmania Donovani / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 820-824, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-294561
ABSTRACT
The objective of this study was to construct and express recombinant prokaryotic plasmid pET32a (+)- ast1 in E. coli BL21(DE3). Amastin gene was amplified from genomic DNA of Leishmania Donovani and its transmembran region was predicted by the methods of SOSUI and Tmpred; astl located in N-terminus of amastin gene was amplified and cloned into prokaryotic plasmid pET32a(+), which was named pET32a(+)-ast1, and then rAST1 was expressed in E. coli BL21(DE3). The results of SDS-PAGE and immunobloting assay showed that a fusion protein rAST1 (relative molecular mass about 27 kDa) was able to express in BL21. The recombinant prokaryotic plasmid pET32a(+)- ast1 was successfully constructed, and noted to be efficiently expressed in E. coli BL21(DE3).
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Leishmania donovani
/
Recombinant Fusion Proteins
/
Protozoan Proteins
/
Cloning, Molecular
/
Genes, Protozoan
/
Escherichia coli
/
Extracellular Space
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2009
Type:
Article
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