Morphological Changes of Retinal Vessel Under Tissue Culture
Journal of the Korean Ophthalmological Society
;
: 1287-1292, 2002.
Article
in Korean
| WPRIM
| ID: wpr-29460
ABSTRACT
PURPOSE:
This study was performed to investigate the growth pattern of the retinal vessel including retinal vascular endothelial cell and pericyte.METHODS:
The sensory retina was detached from the eyecup obtained from donor's eye. The retinal vessel which was separated from the sensory retina was cultured in tissue culture media for 2, 3 and 4 weeks separately and examined by transmission electron microscopy.RESULTS:
On the second week of tissue culture, both the retinal vascular endothelial cells and pericyte were intact in morphology in nuclear and cytoplasmic pattern. Both cells were partially detached from the surrounding basement membrane. On the third week of tissue culture, the cytoplasm of the retinal vascular endothelial cells and pericyte were degenerated, whereas the vascular endothelial cell were intact in nuclear and cytoplasmic profiles which were still partially surrounded by the basement membrane.CONCLUSIONS:
These findings suggest that the survivability of the retinal vascular endothelial cells and pericyte may be limited for 2 and 3 weeks, separatedly under the ordinary culture medium.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Retina
/
Retinal Vessels
/
Retinaldehyde
/
Basement Membrane
/
Pericytes
/
Culture Media
/
Cytoplasm
/
Endothelial Cells
/
Microscopy, Electron, Transmission
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2002
Type:
Article
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