TIMP-1 secreted by fibroblasts inhibits tumor cell invasion and metastasis in mouse melanoma / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 610-614, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-294607
ABSTRACT
We constructed a recombinant adenoviral vector expressing human tissue inhibitors of metalloproteinase-1(TIMP-1), and evaluated the inhibition of TIMP-1 secreted by primary fibroblasts after infection with adenovirus-mediated TIMP-1 gene (Ad-TIMP-1) on tumor cell invasion and metastasis in mouse melanoma. It was found that TIMP-1 was detected in the supernatants of cultured mouse primary fibroblasts after infection with Ad-TIMP-1 by indirect enzyme-linked immunosorbent assay (ELISA). The TIMP-1 secreted by Ad-TIMP-1 infected primary fibroblast significantly inhibited B16BL6 cell invasion and metastasis both in vitro and in vivo. We also demonstrated that the primary fibroblasts transfected by Ad-TIMP-1, after being subcutaneously injected into mouse, can secreted TIMP-1 into the blood of mouse and maintained at the therapeutic in vivo levels of TIMP-1. These results suggest that the preparation of Ad-TIMP-1 infected primary fibroblast be an effective method to deliver TIMP-1 gene in vivo, which provides a new strategy of gene therapy and has the potential for clinical applications in the treatment of tumor cell metastasis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Therapeutics
/
Melanoma, Experimental
/
Recombinant Proteins
/
Genetic Therapy
/
Adenoviridae
/
Tissue Inhibitor of Metalloproteinase-1
/
Fibroblasts
/
Genetics
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2009
Type:
Article
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