Hydrogel self-supported from the neotype of amphiphilic peptide / 生物医学工程学杂志

ABSTRACT

The neotype of amphiphilic oligopeptide (C16 H31 O-AAAGGGGDDIKVAV) was synthesized. The framework of three-dimensional and porous hydrogel self-assembly from the amphiphilic oligopeptide on different conditions was explored. The peptide, whose molecular weight (MW) and purity were detected by Mass Spectrometer (MS) and High Performance Liquid Chromatograph (HPLC) respectively, was synthesized in solid phase methods. Peptide was dissolved in 0.1 mol/L Sodium Hydroxide (NaOH) solution. 200 microl of 10, 2, 1, 0.5 wt% peptide solutions, which were prepared respectively, were added into the same volume of DMEM/F12, or placed into the vapor of 10 mol/L Hydrochloric acid (HCl), or were used to coat in the surface of coverslip and set into the baking oven at 37 degrees C. The self-assembly hydrogel was examined with transmission electron microscope (TEM) and scanning electron microscope (SEM). MS showed that peptide MW was 1438.31. HPLC testified that the peptide purity was 96%. The peptide solution was self-supported into hydrogel triggered with DMEM/F12 in few seconds, or the thin hydrogel after two hours in the vapor of 10 mol/L HCl, or not hydrogel in the baking oven at 37 degrees C. SEM showed that the hydrogel self-assembly from 10 wt% peptide solution was composed of nanofibers that ranked in layers where there were thick voids. TEM showed that the hydrogel self-assembly from 2, 1, 0.5 wt% peptide solution comprised woven network nanofibers, that the nanofibers of hydrogel self-supported from 1 wt% peptide solution varied from 3 to 6 nm in diameter and 100 nm to 1.5 um in length, that the nanofibers of hydrogel self-supported from 2 wt% peptide solution ranked closely, and there were big voids within the thin nanofibers of hydrogel self-supported from 0.5 wt% peptide solution. The amphiphilic oligopeptide was synthesized and self-organized successfully into porous hydrogel characterized as "intelligent" tissue engineering scaffolds containing the bioactive ligand, which was triggered by DMEM/F12.