Identification of differentially expressed genes in myocardium of patients with heart failure by human whole genomic oligonucleotide microarray-assisted pathways analysis / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 120-125, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-294766
ABSTRACT
<p><b>OBJECTIVE</b>To identify the differentially expressed gene profiles in myocardium of patients with heart failure using human whole genomic oligonucleotide microarray-assisted pathway analysis.</p><p><b>METHODS</b>Phalanx whole genomic oligonucleotide microarrays were used to detect the gene expression profiles of myocardium in four patients died of heart failure and 4 brain died patients without heart diseases. The microarray findings were confirmed by real-time quantitative reverse transcriptase-polymerase chain reaction. The genes with a threshold of 1.2 times fold-change were selected and BioCarta Pathway and KEGG (Kyoto Encyclopaedia of Genes and Genomes) pathway databases were used to identify functionally related gene pathways.</p><p><b>RESULTS</b>A total of 2806 genes with differentially expression were detected between the failing and non-failing heart samples, expression changes of 399 genes were more than 2-folds. Eleven pathways were identified by BioCarta pathway database and sixteen pathways were identified by KEGG PATHWAY Database.</p><p><b>CONCLUSION</b>Genomic microarray-assisted pathway analysis could help to identify gene expression profiles in failing heart.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA
/
Signal Transduction
/
Genome, Human
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
Genome-Wide Association Study
/
Genes
/
Genetics
/
Genotype
/
Heart Failure
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2009
Type:
Article
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