Identification of hydroxylate metabolites of daidzein and its sulfate conjugates in rat urine by LC-ESI/MS(n) / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1004-1009, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-294898
ABSTRACT
<p><b>AIM</b>To identify the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine.</p><p><b>METHODS</b>Urine samples from 0 - 24 h were collected after single ig dose of 500 mg x kg(-1) daidzein to each of six rats. The urine samples were purified by SPE column (SPE C18) and analyzed with liquid chromatographic-tandem electrospray ionization ion trap mass spectrometry (LC-ESI/MS(n)) for potential metabolites.</p><p><b>RESULTS</b>Several new hydroxylate metabolites and its sulfate conjugates were found and identified in rat urine.</p><p><b>CONCLUSION</b>LC-ESI/MS(n) is proved to be a simple, rapid, sensitive and specific technique for identification of the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Seeds
/
Glycine max
/
Sulfates
/
Urine
/
Molecular Structure
/
Chemistry
/
Reproducibility of Results
/
Chromatography, Liquid
/
Rats, Sprague-Dawley
/
Spectrometry, Mass, Electrospray Ionization
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2006
Type:
Article
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