Determination in vitro of rat plasma protein binding rate of sinomenine by using microdialysis method / 药学学报
Acta Pharmaceutica Sinica
;
(12): 909-912, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-294915
ABSTRACT
<p><b>AIM</b>To determine in vitro the rat plasma protein binding rate by using microdialysis method.</p><p><b>METHODS</b>The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method. The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate. The x-intercept of regression equation was the free drug concentration (Cf). The plasma protein binding rate was calculated by using the following equation f = ( C0 - Cf)/C0.</p><p><b>RESULT</b>The binding rate was kept relatively stable in the studied concentration range.</p><p><b>CONCLUSION</b>It is feasible that the plasma protein binding rate can be determined by using microdialysis method.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Protein Binding
/
Blood Proteins
/
Chemistry
/
Regression Analysis
/
Chromatography, High Pressure Liquid
/
Rats, Sprague-Dawley
/
Microdialysis
/
Sinomenium
/
Metabolism
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2006
Type:
Article
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