Separation and identification of Taxol in the crude extracts of Taxus cuspidata and its callus culture with HPLC-ESI-MS/MS / 药学学报
Acta Pharmaceutica Sinica
;
(12): 863-866, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-294924
ABSTRACT
<p><b>AIM</b>To study the MS/MS fragmentation mechanism of Taxol, and based on it to establish HPLC-ESI-MS/MS technique to separate and identify Taxol in the crude extracts of Taxus cuspidata and its callus culture, consequently to provide a fast and credible method for the analysis of Taxol in natural products.</p><p><b>METHODS</b>Optimized the HPLC-ESI-MS/MS parameters for the sample analysis, and then discussed the ionization and cleavage mechanism of Taxol in ESI-MS and ESI-MS/MS, finally identified the Taxol in the samples with retention time, molecular weight and MS/MS spectra.</p><p><b>RESULTS</b>Elucidated the MS/MS fragmentation mechanism of Taxol, and developed HPLC-ESI-MS/MS method to analyze Taxol in the two samples.</p><p><b>CONCLUSION</b>The HPLC-ESI-MS/MS method is rapid, highly sensitive and specific, so it is suitable for the separation and identification of Taxol in natural products.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Plant Extracts
/
Chemistry
/
Reproducibility of Results
/
Chromatography, Liquid
/
Paclitaxel
/
Taxus
/
Spectrometry, Mass, Electrospray Ionization
/
Tandem Mass Spectrometry
/
Methods
Type of study:
Diagnostic study
/
Prognostic study
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2006
Type:
Article
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