Liquid chromatography-tandem electrospray ionization ion trap mass spectrometric assay for the metabolites of jatrorrhizine in rat urine / 药学学报
Acta Pharmaceutica Sinica
;
(12): 846-851, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-294927
ABSTRACT
<p><b>AIM</b>To identify the main metabolites of jatrorrhizine in rat urine.</p><p><b>METHODS</b>The rat urine samples were collected 0 - 72 h after ig 12 mg x kg(-1) jatrorrhizine, then the samples were purified through C18 solid-phase extraction cartridge. The purified samples were analyzed by combining liquid chromatography and tandem electrospray ionization ion trap mass spectrometry (LC-ESI/ITMS(n)). Identification and structural elucidation of the metabolites were performed by comparing the changes in molecular masses, retention-times and full scan MS(n) spectra with those of the parent drug.</p><p><b>RESULTS</b>At least seven phase I metabolites (such as de-methyl, de-hydrogen and hydroxyl metabolites) and eleven phase II metabolites (such as glucuronide conjugates and methyl-conjugates) were identified in rat urine.</p><p><b>CONCLUSION</b>The developed LC-ESI/ITMS(n) method is not only simple and rapid but also sensitive and specific for the identification of metabolites of jatrorrhizine in rat urine.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Urine
/
Berberine
/
Molecular Structure
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Rats, Wistar
/
Spectrometry, Mass, Electrospray Ionization
/
Coptis
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2006
Type:
Article
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