Peptide mapping analysis of recombinant human interleukin-11 with HPLC-ESI-Q-TOF/MS spectrometry / 药学学报
Acta Pharmaceutica Sinica
;
(12): 756-760, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-294944
ABSTRACT
<p><b>AIM</b>To analyze the peptide mapping of recombinant human interleukin-11 (rhIL-11) by HPLC-ESI-Q-TOF/MS spectrometry.</p><p><b>METHODS</b>The trypsin digested rhIL-11 at 37 degrees C over night, and the peptide mapping was performed by HPLC. The relative molecular weight of the peptides fragments was measured by ESI-Q-TOF/MS, and amino acid sequence was analyzed by MS/MS.</p><p><b>RESULTS</b>The peptide fragments of rhIL-11 in the peptide mapping were assigned by analyzing the retain time, relative molecular weight and amino acid sequence. And 97% of the expected peptides were detected in this way.</p><p><b>CONCLUSION</b>The study proves that HPLC-ESI-Q-TOF/MS is a good method to analyze peptide mapping of protein with the advantage of sensitivity, high speed and accuracy.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptide Fragments
/
Peptide Mapping
/
Recombinant Proteins
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Amino Acid Sequence
/
Interleukin-11
/
Spectrometry, Mass, Electrospray Ionization
/
Tandem Mass Spectrometry
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS