Auto-dendritic cell vaccines pulsed with PSA, PSMA and PAP peptides for hormone-refractory prostate cancer / 中华男科学杂志
National Journal of Andrology
; (12): 698-704, 2010.
Article
in Zh
| WPRIM
| ID: wpr-295015
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the clinical safety and effects of auto-dendritic cells pulsed with HLA-A201-binding peptides prostate-specific antigen (PSA) , prostate-specific membrane antigen (PSMA) and prostatic acid phosphatase (PAP) in the treatment of hormone-refractory metastatic prostate cancer (HRPC).</p><p><b>METHODS</b>Sixteen HRPC patients with positive HLA-A201 were enrolled and their monocytes isolated and induced into dendritic cells with the combination of rhGM-CSF and rhIL4. The patients were inoculated subcutaneously near the inguinal region with auto-DCs pulsed with peptides PSA (KLQCVDLHV) , PSMA (ALDVYNGL L) and PAP (LLHETDSAV) every 2 weeks for 4 times, and the immunological and clinical responses were examined within 1 -2 weeks after the final vaccination.</p><p><b>RESULTS</b>Vaccination of dendritic cells was well tolerated and no toxicity was observed. The cytokine levels in the serum such as IL-2, IL-12 and IFN-gamma were significantly increased after the vaccination (P < 0.01). The delayed type hyper- sensitivity (DTH) test was positive in 4 of the patients (4/11), the percentage of antigen-special IFN-gamma+ CD8+ T increased in 5 (5/11), the level of the tumor marker PSA decreased in 6 (6/16) , hydrops abdominis reduced in 1 (1/16), and the size of the cervical lymph node lessened in 1 (1/16). Three patients showed partial remission (PR), 7 stability of the disease (SD), and the other 6 progression of the disease (PD).</p><p><b>CONCLUSION</b>Auto-DC vaccines loaded with PSA, PSMA and PAP peptides, capable of eliciting specific immune responses in HRPC patients, is a safe and effective option for the treatment of advanced HRPC.</p>
Full text:
1
Index:
WPRIM
Main subject:
Prostatic Neoplasms
/
Therapeutics
/
Acid Phosphatase
/
Blood
/
Dendritic Cells
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HLA-A2 Antigen
/
Cytokines
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Treatment Outcome
/
Protein Tyrosine Phosphatases
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Prostate-Specific Antigen
Limits:
Aged
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Humans
/
Male
Language:
Zh
Journal:
National Journal of Andrology
Year:
2010
Type:
Article