Angiotensin II induced cardiac hypertrophy is blocked by PTEN via suppressing Ca2+/Calcineurin pathway / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 541-545, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-295279
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of PTEN on Ang II induced cardiomyocyte hypertrophy and subsequent Ca(2+)/Calcineurin pathway changes.</p><p><b>METHODS</b>Primary cultured neonatal rat cardiomyocytes were cultured and were treated with phosphate-buffered saline, empty adenovirus (Ad-GFP), or adenovirus encoding for PTEN (Ad-PTEN-GFP) for 48 h and Ang II (10(-7) mol/L) was added to the medium for another 24 h. Cells were harvested and intracellular Ca(2+) concentration ([Ca(2+)] i) was determined by Fura-2/AM ratio imaging analysis; PTEN, ANF, beta-MHC and CaNAbeta mRNA evaluated with RT-PCR; PTEN and CaNAbeta protein by Western blot; CaN phosphatase activity by CaN detecting kits.</p><p><b>RESULTS</b>PTEN at mRNA and protein levels were significantly higher in Ad-PTEN-GFP treated cardiomyocytes than that of Ad-GFP treated cardiomyocytes. Ang II stimulation upregulated [Ca(2+)] i, CaNAbeta at mRNA and protein levels and CaN phosphatase activity in Ad-GFP treated cardiomyocytes but not in Ad-PTEN-GFP treated cardiomyocytes.</p><p><b>CONCLUSIONS</b>Cardiac hypertrophy induced by Ang II could be blocked by PTEN overexpression via suppressing Ca(2+)/Calcineurin pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Angiotensin II
/
Signal Transduction
/
Cells, Cultured
/
Adenoviridae
/
Calcium
/
Rats, Wistar
/
Cardiomegaly
/
DNA, Complementary
/
Calcineurin
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2006
Type:
Article
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