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Sarcolemmal sodium-calcium exchanger mediated the beneficial effects of myocardial ischemic preconditioning and pharmacological preconditioning / 中华心血管病杂志
Chinese Journal of Cardiology ; (12): 367-371, 2006.
Article in Chinese | WPRIM | ID: wpr-295314
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of sodium-calcium exchanger (NCX) on ischemic preconditioning and pharmacological preconditioning.</p><p><b>METHODS</b>Cultured rat neonatal cardiomyocytes were randomly divided into 6 groups (1) ischemia/reperfusion group (9 h ischemia followed by 1 h reperfusion, I/R), (2) ischemic preconditioning group (1.5 h ischemia/1 h reperfusion + I/R), (3) pharmacologic preconditioning group, adenosine (10 micromol/L) pretreated for 1 h + I/R, (4) calmodulin-dependent protein kinase II (CaMKII) inhibitor KN-93 (0.5 micromol/L for 0.5 h) + ischemic preconditioning group, (5) KN-93 + pharmacologic preconditioning group, (6) control group. The leakage of intracellular lactate dehydrogenase (LDH) in various groups was determined by biochemical autoanalyzer. Semi-quantitative RT-PCR was employed to measure the mRNA levels of sodium-calcium exchanger. Activity of sodium-calcium exchanger (Na(+)-dependent (45)Ca(2+) uptake) was measured by liquid scintillation counting.</p><p><b>RESULTS</b>(1) Compared to the I/R group, the LDH leakages in both ischemic preconditioning group and pharmacologic preconditioning group were significantly reduced (P < 0.05) while significantly increased in the KN-93 + pharmacologic preconditioning group and the KN-93 + ischemic preconditioning group (P < 0.05). (2) The Na(+)-dependent (45)Ca(2+) uptake was significantly increased in the I/R group (P < 0.05) compared to control group and this increase could be significantly attenuated in ischemic preconditioning group and adenosine pretreatment group (P < 0.05). (3) The expression of NCX mRNA in I/R group was also significantly increased (P < 0.05) in the I/R group (P < 0.05) compared to control group and this increase could be significantly attenuated in ischemic preconditioning group and adenosine pretreatment group (P < 0.05), CaMKII inhibitor KN-93 significantly abolished these effects in preconditioning group (P < 0.05) and in adenosine pretreated group (P < 0.05).</p><p><b>CONCLUSION</b>NCX mediated the cardioprotective effects of ischemic preconditioning and pharmacological preconditioning in the neonatal cardiomyocytes I/R model.</p>
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Therapeutics / RNA, Messenger / Myocardial Reperfusion Injury / Cells, Cultured / Calcium / Rats, Sprague-Dawley / Ischemic Preconditioning, Myocardial / Sodium-Calcium Exchanger / Myocytes, Cardiac / Calcium-Calmodulin-Dependent Protein Kinase Type 2 Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Chinese Journal of Cardiology Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Therapeutics / RNA, Messenger / Myocardial Reperfusion Injury / Cells, Cultured / Calcium / Rats, Sprague-Dawley / Ischemic Preconditioning, Myocardial / Sodium-Calcium Exchanger / Myocytes, Cardiac / Calcium-Calmodulin-Dependent Protein Kinase Type 2 Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Chinese Journal of Cardiology Year: 2006 Type: Article