Induction of Nestin Early Expression as a Hallmark for Mesenchymal Stem Cells Expression of PDX-1 as a Pre-disposing Factor for Their Conversion into Insulin Producing Cells
International Journal of Stem Cells
;
: 76-82, 2017.
Article
in English
| WPRIM
| ID: wpr-29538
ABSTRACT
Diabetes constitutes a worldwide epidemic that affects all ethnic groups. Cell therapy is one of the best alternatives of treatment, by providing an effective way to regenerate insulin-producing cells lost during the course of the disease, but many issues remain to be solved. Several groups have been working in the development of a protocol capable of differentiating Mesenchymal Stem Cells (MSCs) into physiologically sound Insulin Producing Cells (IPCs). In order to obtain a simple, fast and direct method, we propose in this manuscript the induction of MSCs to express NESTIN in a short time period (2 h), proceeded by incubation in a low glucose induced medium (24 h) and lastly by incubation in a high glucose medium. Samples from cell cultures incubated in high glucose medium from 12 to 168 h were obtained to detect the expression of INSULIN-1, INSULIN -2, PDX-1 and GLUT-2 genes. Induced cells were exposed to a glucose challenge, in order to assess the production of insulin. This method allowed us to obtain cells expressing PDX-1, which resembles a progenitor insulin-producing cell.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ethnicity
/
Cell Culture Techniques
/
Mesenchymal Stem Cells
/
Nestin
/
Cell- and Tissue-Based Therapy
/
Glucose
/
Insulin
/
Methods
Type of study:
Practice guideline
Limits:
Humans
Language:
English
Journal:
International Journal of Stem Cells
Year:
2017
Type:
Article
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