Full sequence analysis for a null allele of MICA gene (MICA*063N) / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the full nucleotide sequence of a null allele of major histocompatibility complex class I chain-related gene (MICA).</p><p><b>METHODS</b>A sequence-based typing method was used to determine the nucleotide sequence of the MICA gene. Potential alleles were identified with a computer program.</p><p><b>RESULTS</b>The identified allele has possessed a sequence similar to that of MICA*027 except for a C→T substitution at position 184 in codon 62 (CAG→TAG) of exon 2. As a stop codon, this may result in a truncated protein.</p><p><b>CONCLUSION</b>A null allele of MICA gene has been identified. The sequence has been submitted to the Genbank nucleotide sequence database (submission No. HWS10011131), which was officially named as MICA*063N by the WHO Nomenclature Committee in October 2010.</p>