Detection and analysis of partial sequences isolated from human and swine in rural area of southern China / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 984-987, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-295643
ABSTRACT
<p><b>OBJECTIVE</b>To study the genotype of swines isolated from humans and their relationships with hepatitis E virus (HEV) in the rural areas of southern China.</p><p><b>METHODS</b>Specimens collected from normal people with HEV-IgM positive, acute hepatitis E patients and from swine in the same area were detected for HEV RNA using RT-nPCR with ORF2 primers. The positive PCR products were cloned and sequenced.</p><p><b>RESULTS</b>13 out of the 132 samples from swine stool, 4 of 26 HEV-IgM positive sera of normal people and 1 of 4 acute hepatitis E patients' stool sample and sera were tested positive for HEV RNA. Data from sequence analysis showed that the identity at nucleotide level was 89.3%-100.0% among the 10 isolates which shared 78.7% - 84.7%, 83.3% - 85.3%, 76.0% - 80.0% and 84.7% - 95.3% nucleotide sequence identity with HEV genotype I, II, III and IV respectively in the region (nt6317- 6466).</p><p><b>CONCLUSION</b>HEV circulating in humans and swine in the area belonged to genotype IV.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Swine
/
Swine Diseases
/
Virology
/
Blood
/
Immunoglobulin M
/
RNA, Viral
/
China
/
Rural Health
/
Hepatitis E virus
/
Hepatitis E
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Epidemiology
Year:
2005
Type:
Article
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