Detection of leukemia-derived microparticles in the monitoring of chronic myeloid leukemia / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 138-141, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-295692
ABSTRACT
<p><b>OBJECTIVE</b>To exam the role of leukemia cells-derived microparticles in the post-complete molecular response stratification.</p><p><b>METHODS</b>Blood samples from 29 patients diagnosed with chronic myeloid leukemia (CML) were collected. Microparticles (MP) were extracted from the peripheral blood. Real-time PCR was performed to measure the level of BCR-ABL mRNA.</p><p><b>RESULTS</b>BCR-ABL mRNA could be stably detected both in MP and peripheral blood cells; BCR-ABL in MP showed significant difference within complete molecular response, major molecular response and complete cytogenetic response (9.1±2.8, 25.2±6.9 and 62.8±6.3 respectively, P<0.05). BCR-ABL was detected in MP even when it was negative in peripheral blood cells (3.7-15.3). For patients with complete molecular response, BCR-ABL in MP but not cells were significantly different between imatinib and stem cell transplant recipients (3.3±2.1 vs 9.1±2.8, P<0.05).</p><p><b>CONCLUSION</b>This study indicated that MP may serves as a new target for monitoring of CML. Quantification of BCR-ABL in MP may offer a novel strategy for stratification of molecular response.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
RNA, Messenger
/
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
/
Fusion Proteins, bcr-abl
/
Cell-Derived Microparticles
/
Real-Time Polymerase Chain Reaction
/
Genetics
Type of study:
Diagnostic study
Limits:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS