Application of SCGE-FPG in the study of arsenic-induced oxidative DNA damage in PHA-stimulated and unstimulated human lymphocytes / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 12-15, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-295793
ABSTRACT
<p><b>OBJECTIVE</b>To confirm that arsenic (As) induces oxidative DNA damage in phytohemagglutinin (PHA)-stimulated and unstimulated human lymphocytes.</p><p><b>METHODS</b>The alkaline comet assay combined with specific enzyme (Formamidopyrimidine-DNA glycosylase, FPG) digestion was used to measure As-induced base damage.</p><p><b>RESULTS</b>The enzyme-sensitive sites were readily detected with the alkaline comet assay after the cells were treated with 10 micromol As for 2 hours. The repair patterns observed for FPG-created DNA single strand breaks (SSBs) in As-treated cells were comparable to those in hydrogen peroxide (H(2)O(2))-treated cells. The enzyme-created SSBs, As-induced base damage, were more significantly revealed in PHA-stimulated lymphocytes. About 63% and 68% of SSBs induced by As and H(2)O(2), respectively, were repaired in PHA-stimulated lymphocytes by 2-hour repair incubation, but about 34% and 43%, respectively, were repaired in unstimulated cells. About 40% and 49% of base damage induced by As and H(2)O(2), respectively, were repaired in PHA-stimulated lymphocytes, but about 19% and 21 %, respectively, were repaired in unstimulated cells.</p><p><b>CONCLUSIONS</b>As induces oxidative DNA damage in human lymphocytes within micromolar concentrations. Like the damage induced by H(2)O(2), As-induced DNA damage was more slowly repaired in unstimulated lymphocytes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxidation-Reduction
/
Pharmacology
/
Phytohemagglutinins
/
Arsenic
/
DNA Damage
/
DNA, Single-Stranded
/
Lymphocytes
/
DNA-Formamidopyrimidine Glycosylase
/
DNA Repair
/
Electrophoresis
Limits:
Adult
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2002
Type:
Article
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