Effects of a regional Chinese diet and its vitamin supplementation on proliferation of human esophageal cancer cell lines / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 442-448, 2008.
Article
in English
| WPRIM
| ID: wpr-296026
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of a local diet popular in Yanting region (YT diet) on the proliferation of two human cell lines (Eca-109 esophageal squamous cell carcinoma line and HL7702 normal liver epithelial cell line) in rats by a sero-physiological approach.</p><p><b>METHODS</b>Male SD rats were divided into six groups and fed respectively with a conventional diet and the YT diet (one of the five experimental diets) supplemented with two vitamin mixtures (Mix. 1 vitamins A, E, and folic acid; Mix.2 mix.1 plus riboflavin and vitamin C) at two different doses. On the 30th day, sera were collected from the rats and added into a medium for cell culture, with 10% FBS used as a serum control. The effects were assessed by MTT assay, DNA synthesis and flow cytometry assays.</p><p><b>RESULTS</b>Compared with the control, the sera from rats fed with the YT diet significantly promoted the proliferation of Eca-109 cells, which was, however, reversed by the supplementation with two vitamin mixtures at high doses. Surprisingly, the same treatment produced contrary effects on HL7702 cells as compared with Eca-109 cells.</p><p><b>CONCLUSION</b>The sera from rats fed with the YT diet could promote the proliferation of human esophageal cancer cell line Eca-109, whereas the sera from those fed with the YT diet supplemented with vitamin mixtures might have inhibitory effects on the proliferation of Eca-109 cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Time Factors
/
Vitamins
/
Esophageal Neoplasms
/
Carcinoma, Squamous Cell
/
Weight Gain
/
China
/
Rats, Sprague-Dawley
/
Dietary Supplements
/
Cell Biology
Limits:
Animals
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2008
Type:
Article
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