Tissue extracts from infarcted myocardium of rats in promoting the differentiation of bone marrow stromal cells into cardiomyocyte-like cells / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 110-117, 2008.
Article
in English
| WPRIM
| ID: wpr-296076
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether cardiac tissue extracts from rats could mimic the cardiac microenvironment and act as a natural inducer in promoting the differentiation of bone marrow stromal cells (BMSCs) into cardiomyocytes.</p><p><b>METHODS</b>Three kinds of tissue extract or cell lysate [infarcted myocardial tissue extract (IMTE), normal myocardial tissue extract (NMTE) and cultured neonatal myocardial lysate (NML)] were employed to induce BMSCs into cardiomyocyte-like cells. The cells were harvested at each time point for reverse transcription-polymerase chain reaction (RT-PCR) detection, immunocytochemical analysis, and transmission electron microscopy.</p><p><b>RESULTS</b>After a 7-day induction, BMSCs were enlarged and polygonal in morphology. Myofilaments, striated sarcomeres, Z-lines, and more mitochondia were observed under transmission electron microscope. Elevated expression levels of cardiac-specific genes and proteins were also confirmed by RT-PCR and immunocytochemistry. Moreover, IMTE showed a greater capacity of differentiating BMSCs into cardiomyocyte-like cells.</p><p><b>CONCLUSIONS</b>Cardiac tissue extracts, especially IMTE, can effectively differentiate BMSCs into cardiomyocyte-like cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bone Marrow Cells
/
Immunohistochemistry
/
Base Sequence
/
Cell Differentiation
/
Stromal Cells
/
Rats, Sprague-Dawley
/
DNA Primers
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Biology
/
Microscopy, Electron, Transmission
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2008
Type:
Article
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