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The Isolation and Identification of Infectious Bronchitis Virus PTFY Strain in Muscovy Ducks / 病毒学报
Chinese Journal of Virology ; (6): 203-209, 2016.
Article in Zh | WPRIM | ID: wpr-296196
Responsible library: WPRO
ABSTRACT
In July 2009, some farms of breeding Muscovy ducks on the peak of egg laying suffered the decrease of hatching rate and the quality of the eggs showing low mortality and no evident respiratory symptoms. The swelling and congestive ovary was visible after autopsy. This study was brought out for the diagnosis of these cases. The virus was isolated and identified by the methods of virus culture in chicken embryo, physical and chemical properties test, hemagglutinin test, NDV (Newcastle diseases Virus) interference test, electron microscope observation, pathogenicity test and the gene sequence analysis. The results indicated the virus showed the characters of inducing dwarf embryo after inocubation, the sensibility to lipid solvent and the hemagglutination capacity after pancreatic enzyme treatment, the typical morphology of coronavirus, the interference to NDV replication and the homology among 84.7% - 99% of the particial N gene sequences to the reference IBV (Avian infectious bronchitis virus) strains. The strain was identified as IBV isolate and this study confirmed the pathogenicity of IBV to Muscovy ducks.
Subject(s)
Full text: 1 Index: WPRIM Main subject: Phylogeny / Poultry Diseases / Virology / Molecular Sequence Data / Sequence Alignment / Amino Acid Sequence / Classification / Coronavirus Infections / Infectious bronchitis virus / Ducks Limits: Animals Language: Zh Journal: Chinese Journal of Virology Year: 2016 Type: Article
Full text: 1 Index: WPRIM Main subject: Phylogeny / Poultry Diseases / Virology / Molecular Sequence Data / Sequence Alignment / Amino Acid Sequence / Classification / Coronavirus Infections / Infectious bronchitis virus / Ducks Limits: Animals Language: Zh Journal: Chinese Journal of Virology Year: 2016 Type: Article