Generation of Japanese Encephalitis Virus-like Particle Vaccine and Preliminary Evaluation of Its Protective Efficiency / 病毒学报
Chinese Journal of Virology
;
(6): 150-155, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-296204
ABSTRACT
The cDNA fragment of JEV prME gene was cloned into the baculovirus shuttle vector (bacmid) to construct a recombinant baculovirus vector, defined as AcBac-prME. Then the recombinant baculovirus Ac-prME was obtained by transfecting Sf9 cells with AcBac-prME. Western blot analysis and immunofluorescence results indicated that both prM and E proteins were efficiently expressed in Sf9 cells. Electron microscopy suggested that prME was assembled into JEV-VLPs. To further evaluate the potential of JEV-VLPs as vaccine, the mice were immunized with JEV-VLPs and then challenged with lethal JEV. The results of mice survival and pathological changes demonstrated that the JEV-VLPs performed complete protection against JEV-P3 strain and relieved pathological changes in the mice brain significant. This study suggest that JEV-VLPs would be a potential vaccine for Japanese encephalitis virus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Viral Envelope Proteins
/
Vaccination
/
Encephalitis, Japanese
/
Japanese Encephalitis Vaccines
/
Encephalitis Virus, Japanese
/
Allergy and Immunology
/
Vaccines, Virus-Like Particle
/
Sf9 Cells
/
Genetics
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS