Mechanism Underlying Increased Expression of a Member of the Serine/Threonine Kinase Family (Citron kinase) Induced by HIV-1 Infection / 病毒学报
Chinese Journal of Virology
;
(6): 388-394, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-296272
ABSTRACT
Human immunodeficiency virus (HIV)-1 infection changes transcriptional profiles and regulates. the factors and machinery of the host that facilitate viral replication. Our previous study suggested that the serine/threonine kinase citron kinase (citK) promotes HIV-1 egress. To ascertain if HIV-1 infection affects citK expression in primary cells, peripheral blood mononuclear cells were infected with vesicular stomatitis virus G protein (VSV-G)-pseudotyped HIV-1 vector NL4-3-luc viruses, which resulted in remarkably increased expression of citK. citK overexpression led to a more than two-fold increase in HIV-1 production, whereas a significant decrease was observed when citK was depleted in CD4+ T cells. Infection with HIV-1 pseudoviruses induced increases in the mRNA and protein levels of citK by 2. 5- and 2. 7-fold in HEK293T cells, respectively. By cloning the 5-kb promoter of citK into a luciferase reporter system and transfecting the construct into HEK293T cells, enhanced luciferase activity was observed during HIV-1 infection. Taken together, these data demonstrate that HIV-1 infection upregulates citK expression at the transcriptional level, and thereby renders the host more susceptible to invasion by HIV-1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Virology
/
Virus Replication
/
CD4-Positive T-Lymphocytes
/
Gene Expression Regulation, Enzymologic
/
Up-Regulation
/
HIV-1
/
Cloning, Molecular
/
Protein Serine-Threonine Kinases
/
Intracellular Signaling Peptides and Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2015
Type:
Article
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