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Sequence and Structural Analyses of the Complete Genome of Bovine Papillomavirus 2 Genotype Aks-01 Strain from Skin Samples of Cows in Southern Xinjiang, China / 病毒学报
Chinese Journal of Virology ; (6): 370-378, 2015.
Article in Chinese | WPRIM | ID: wpr-296274
ABSTRACT
To study the complete genomic sequence, genomic characteristics, and genetic variation of the bovine papillomavirus 2 genotype (BPV-2) Aks-01 strain at the molecular level, genotyping of this strain from the skin samples of cows in southern Xinjiang (China) was first detected by the polymerase chain reaction with FAP59/FAP64 primers. Based on the complete genome of the BPV-2 reference strain, specific primers and sequencing primers were designed, and the complete genome of the Aks-01 strain amplified and sequenced. Sequence analyses showed that genotyping of the Aks-01 strain belonged to BPV-2. The Aks-01 strain had the structural characteristics of BPV-2. The 7944-bp full-length genomic sequence of the Aks-01 strain was compiled using DNAStar™. The sequence of the Aks-01 strain had 98% similarity to the reference strain from GenBank. The Aks-01 strain was most closely related to BPV-1 and BPV-13. BPV-2, BPV-1 and BPV-13 were grouped within the genus Deltapapillomavirus. The Aks-01 strain is the first BPV-2 strain reported in southern Xinjiang.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Skin / Virology / Molecular Sequence Data / Base Sequence / Chemistry / China / Oncogene Proteins, Viral / Amino Acid Sequence / Genome, Viral Limits: Animals Country/Region as subject: Asia Language: Chinese Journal: Chinese Journal of Virology Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Skin / Virology / Molecular Sequence Data / Base Sequence / Chemistry / China / Oncogene Proteins, Viral / Amino Acid Sequence / Genome, Viral Limits: Animals Country/Region as subject: Asia Language: Chinese Journal: Chinese Journal of Virology Year: 2015 Type: Article