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Effect of Smo SiRNA-mediated Hedgehog Signaling Pathway Inhibition on Palatal Fusion / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 594-598, 2016.
Article in English | WPRIM | ID: wpr-296563
ABSTRACT
We used Smo siRNA to inhibit hedgehog signaling pathway in embryonic day (E) 13 palatal shelves in organ culture. SiRNA 4 was chosen as the most efficient from four synthesized Smo siRNAs. Palatal shelf fusion rate of 4 μg/mL cyclopamine group was the lowest and significantly lower than that of blank control group (P<0.05), and that of siRNA 4 group was also lower than that of blank control group (P=0.183). At 48 h after transfection, Smo protein level of siRNA 4 group was 64.8% lower than that of blank control group (P<0.05), and Gli1 protein level of 4 μg/mL cyclopamine group was 68.9% lower than that of blank control group (P<0.05). Hedgehog signaling pathway inhibition decreased palatal fusion in organ culture, probably owing to downregulation of Smo and Gli1 proteins.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Palate / Signal Transduction / Embryology / RNA, Small Interfering / Kruppel-Like Transcription Factors / Hedgehog Proteins / Zinc Finger Protein Gli3 / Zinc Finger Protein Gli2 / Genetics / Metabolism Limits: Animals Language: English Journal: Biomedical and Environmental Sciences Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Palate / Signal Transduction / Embryology / RNA, Small Interfering / Kruppel-Like Transcription Factors / Hedgehog Proteins / Zinc Finger Protein Gli3 / Zinc Finger Protein Gli2 / Genetics / Metabolism Limits: Animals Language: English Journal: Biomedical and Environmental Sciences Year: 2016 Type: Article