Green Tea Polyphenols Alleviate Autophagy Inhibition Induced by High Glucose in Endothelial Cells / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 524-528, 2016.
Article
in English
| WPRIM
| ID: wpr-296573
ABSTRACT
Bovine aortic endothelial cells (BAECs) were cultured with high glucose (33 mmol/L), 4 mg/L green tea polyphenols (GTPs) or 4 mg/L GTPs co-treatment with high glucose for 24 h in the presence or absence of Bafilomycin-A1 (BAF). We observed that high glucose increased the accumulation of LC3-II. Treatment with BAF did not further increase the accumulation of LC3-II. Results also showed an increased level of p62 and decreased Beclin-1. However, GTPs showed inversed trends of those proteins. Furthermore, GTPs co-treatment with high glucose decreased the level of LC3-II and a much higher accumulation of LC3-II was observed in the presence of BAF in comparison with high glucose alone. Results also showed a decreased p62 and increased Beclin-1. The results demonstrated that GTPs alleviated autophagy inhibition induced by high glucose, which may be involved in the endothelial protective effects of green tea against hyperglycemia.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Autophagy
/
Tea
/
Cells, Cultured
/
Chemistry
/
Gene Expression Regulation
/
Macrolides
/
Endothelial Cells
/
Toxicity
/
Polyphenols
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2016
Type:
Article
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