Applicability of the multiplex quantitative antibody array system for early diagnosis of hepatocellular carcinoma / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 785-788, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-296814
ABSTRACT
<p><b>OBJECTIVE</b>To develop an early and accurate detection method for hepatocellular carcinoma (HCC) based on detection of tumor-associated serum markers using a multiplex quantitative antibody array.</p><p><b>METHODS</b>The double-antibody sandwich principle was used to establish an antibody array composed of eight cancer-related serum markers, including alpha-fetoprotein (AFP), hepatocyte growth factor (HGF), insulin-like growth factor (IGF), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), transforming growth factor-beta 1 (TGF-b1), and vascular endothelial growth factor (VEGF). Serum samples from 160 cases of clinically diagnosed HCC and from 58 cases of liver cirrhosis (LC; controls) were obtained to test the array. Sixty percent of the samples were randomly selected for use as the training set (HCC, n = 96; LC, n = 36), and the remaining 40% was used as the test set (HCC, n = 64; LC, n = 22). The SPSS statistical software was used to perform logistic regression analysis and to create a diagnostic model.</p><p><b>RESULTS</b>When used with the training set, the model had sensitivity of 93.3%, specificity of 83.3%, and accuracy of 90.9%. When used with the test set, the model had sensitivity of 89.0%, specificity of 77.3%, and accuracy of 86.0%. The traditional serum AFP value (cut-off value of 20 ng/mL) showed 70.0% diagnostic sensitivity, 59.0% specificity, and 64.0% accuracy.</p><p><b>CONCLUSION</b>The newly developed multiplex quantitative antibody detection system has high sensitivity and specificity. The diagnostic model with AFP and seven other cancer-related factors was superior to the traditional AFP only approach for early diagnosis of liver cancer, indicating its potential clinical value.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Alpha-Fetoproteins
/
Biomarkers, Tumor
/
Sensitivity and Specificity
/
Carcinoma, Hepatocellular
/
Early Diagnosis
/
Microchip Analytical Procedures
/
Diagnosis
/
Allergy and Immunology
/
Liver Neoplasms
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2012
Type:
Article
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