Preparation and identification of recombinant PTD-maxadilan / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1739-1745, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-296864
ABSTRACT
In order to construct a novel fusion protein PTD-maxadilan (PTD-MAX) that can enter the blood-brain barrier (BBB) efficiently, a new gene encoding PTD-MAX was synthesized and cloned into the expression vector pKYB. The recombinant vector pKYB-PTD-MAX was transformed into Escherichia coli ER2566. The expression of fusion protein consisting of PTD-MAX, intein and chitin binding domain was induced by IPTG and the target PTD-MAX protein was purified using Intein Mediated Purification with an Affinity Chitin-binding Tag system. The molecular weight of PTD-MAX determined by the laser flight mass spectrometry was coherent with its theoretical value. The results of the experiment in vivo indicated that the recombinant PTD-MAX can permeate into BBS and inhibitory effects on the food intake were more significantly than maxadilan (P<0.05). The preparation of PTD-MAX lay the foundation for its further application.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Vasodilator Agents
/
Recombinant Fusion Proteins
/
Molecular Sequence Data
/
Pharmacokinetics
/
Base Sequence
/
Blood-Brain Barrier
/
Protein Structure, Tertiary
/
Insect Proteins
/
Escherichia coli
/
Genetic Vectors
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article
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