Effects of surfactin on proliferation, apoptosis and cytoskeleton in human breast cancer MCF-7 cells / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1705-1710, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-296869
ABSTRACT
We studied the effect of surfactin on cell proliferation, apoptosis and the cytoskeleton in human breast cancer cell line MCF-7 in vitro. The result of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) showed that the surfactin inhibited proliferation of MCF-7 cells in a dose- and time-dependent manner, with IC50 at 48 h of 27.3 micromol/L. Surfactin-induced cell death was considered to be apoptotic by observing the typical apoptotic morphological changes by AO/EB staining. Flow cytometric analysis also demonstrated that surfactin caused time-dependent apoptosis of MCF-7 cells through cell arrest at G2/M phase. Immunofluorescence and Western blotting showed that surfactin significantly suppressed the expression of vimentin, induced the alpha-tubulin depolymerization and rearrangement and then the skeleton system of the cells changed dramatically. Based on our findings, surfactin can significantly inhibit the growth of MCF-7 cells and induce apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Peptides, Cyclic
/
Pharmacology
/
Cytoskeleton
/
Breast Neoplasms
/
Apoptosis
/
Cell Line, Tumor
/
Cell Proliferation
/
Lipopeptides
/
Antineoplastic Agents
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article
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