Development of one step RT-PCR technique for detection of H7 subtype avian influenza / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1658-1663, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-296876
ABSTRACT
According to 45 hemagglutinin (HA) gene sequences of H7 subtype of avian influenza virus (AIV), a pair of specific oligonucleotide primers was designed. We developed one step RT-PCR for detecting AIV subtype H7. Sensitivity to detection of allantoic fluid by one step RT-PCR reached 10(5.5) EID50/mL and detection of swab samples reached 10(3) EID50/mL. We simultaneity detected the tissue and swab samples infected with H7 subtypes AIV by one step RT-PCR and virus isolation method. The results showed that the sensitivity of the assay gave an excellent correlation with the conventional virus isolation method. H1-H15 subtypes of avian influenza and other avian diseases were detected by the one step RT-PCR. The results showed the assays were high specific, without cross-reaction with other subtypes or other avian diseases. Development of one step RT-PCR will provide effective technical support for the rapid diagnosis and surveillance of molecular epidemiology of AIV subtype H7.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Birds
/
Sensitivity and Specificity
/
Classification
/
Hemagglutinin Glycoproteins, Influenza Virus
/
Reverse Transcriptase Polymerase Chain Reaction
/
Influenza A Virus, H7N1 Subtype
/
Influenza in Birds
/
Genetics
/
Methods
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article
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