Your browser doesn't support javascript.
loading
Optimized expression of the L1 protein of human papillomavirus in Hansenula polymorpha / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1516-1523, 2009.
Article in Chinese | WPRIM | ID: wpr-296896
ABSTRACT
The heterologously expressed L1 protein of human papilomavirus 16 can assembly into virus-like particles (VLPs), which has been used as prophylactic vaccine for cervical carcinoma. To express L1 protein in Hansenula polymorpha, we analyzed the codon usage of the native gene of L1 protein and redesigned the encoding sequence according to the codon bias of H. polymorpha. We used assembly PCR to synthesize the native gene HPV16L1-N and the codon optimized gene HPV16L1. The synthesized genes were cloned into pMOXZa-A vector to generate plasmids pMOXZ-HPV16N and pMOXZ-HPV16. The expression cassettes MOXp-HPV16L1(N)-AOXTT were cloned into YEp352 vector and transferred into H. polymorpha. After methanol inducement, the expression of L1 protein in H. polymorpha was detected from the codon optimized gene HPV16L1 rather than the native gene HPVI6L1-N. The parameters for induced cultivation for strain HP-U-16L with HPV16L1 were investigated in shaking flask cultures. After induced cultivation in YPM (pH 7.0) medium supplemented with methanol to a final concentration of 1.0% every 12 h at 37 degrees C for 72 h, the recombinant produced 78.6 mg/L of L1 protein. This work offers the possibility for the production of prophylactic vaccine for cervical carcinoma by H. polymorpha.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pichia / Recombinant Proteins / Codon / Oncogene Proteins, Viral / Cloning, Molecular / Capsid Proteins / Human papillomavirus 16 / Genetic Vectors / Genetics / Metabolism Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2009 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pichia / Recombinant Proteins / Codon / Oncogene Proteins, Viral / Cloning, Molecular / Capsid Proteins / Human papillomavirus 16 / Genetic Vectors / Genetics / Metabolism Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2009 Type: Article