Synthesis and characterization of alginate oligosaccharides produced by Pseudomonas mendocina NK-01 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1366-1370, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-296915
ABSTRACT
In this study, we found that when Pseudomonas mendocina NK-01 accumulated intracellular carbon reserve, medium chain length poly (3-hydroxyalkanoates), it also synthesized extracellular saccharides, alginate oligosaccharides. The high carbon nitrogen ratio of culture medium facilitated alginate oligosaccharides production. We analyzed the structure of alginate oligosaccharide by Ultraviolet-Visible Spectrophotometry, Fourier Transform Infrared Spectroscopy, 1H and 13C of Nuclear Magnetic Resonance, and found that it was compounded in line from beta-D-mannuronic acids and alpha-L-gluronic acids via beta-(1-->4)/ alpha-(1-->4) bonds, which acetylated partly on the 2- and/or 3-hydroxy. In addition, we determined the weight-average molecular weight of alginate oligosaccharides by gel permeation chromatography to be 2054.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligosaccharides
/
Chemistry
/
Glucuronic Acid
/
Pseudomonas mendocina
/
Alginates
/
Genetics
/
Hexuronic Acids
/
Metabolism
/
Molecular Weight
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article
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