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Directed evolution of promoter and cellular transcription machinery and its application in microbial metabolic engineering--a review / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1312-1315, 2009.
Article in Chinese | WPRIM | ID: wpr-296923
ABSTRACT
Directed evolution, which is also called molecular evolution, or artificial evolution, combines random mutagenesis and directed selection. In previous studies, it has been extensively applied for the improvement of enzyme catalytic properties and stability, as well as the expanding of substrate specificity. In recent years, directed evolution was also employed in metabolic engineering of promoters for improving their strength and function, and the engineering of global transcription machinery. These techniques contribute to breeding more tolerant strains against environmental stress, as well as strains with improved fermentation efficiency. In this article, we reviewed the applications of directed evolution in the metabolic engineering of promoters and global transcription machinery. These techniques enabled fine-tuning of gene expression and simultaneous alternation of multiple gene transcription inside the cells, and thus are powerful new tools for metabolic engineering.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Saccharomyces cerevisiae / Transcription, Genetic / Industrial Microbiology / Genetic Engineering / Promoter Regions, Genetic / Directed Molecular Evolution / Genetics / Metabolism / Methods Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Saccharomyces cerevisiae / Transcription, Genetic / Industrial Microbiology / Genetic Engineering / Promoter Regions, Genetic / Directed Molecular Evolution / Genetics / Metabolism / Methods Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2009 Type: Article